Trichoderma asperelloides
Authors |
Samuels 2010 |
Strain |
18035 |
Classification |
Hypocreales, Hypocreaceae, Trichoderma |
Culture collection |
processing |
Detection frequency |
Low |
Figure |
Fig. 1 5-day-old colony on PDA Fig. 2 Trichoderma asperelloides. a. Sporulation on OA. b–d. Conidiophores, conidiogenous cells and conidia. e. Conidia. f. Chlamydospore (Bars= 5 μm, unless otherwise specified) |
Colonies |
Colonies on PDA attaining >90-mm in 5 days at 25 °C, raised, cottony, pale yellow, with sparse radial furrows, producing brown, sticky exudate and diffuse yellow pigment with age. Dark green, slimy conidial mass produced on the surface after 7 days. |
Conidiophores |
Conidiophores macronematous, hyaline, erect, with extensively, sometimes verticillately rebranching at right angle, slightly swollen at the branching points, 3.5–5 µm wide near the base of main axis. |
Conidiogenous cells |
Conidiogenous cells phialidic, hyaline, lageniform, laterally or terminally, solitary or in whorls of 2–5, 7–15 × 2.5–3.5 µm. |
Conidia |
Conidia globose to obovoid, slightly pigmented, dark green in mass, 3.0–4.0(–4.5) µm. |
Chlamydospores |
Chlamydospores abundant on OA after 7 days, globose to subglobose, borne terminally, solitary, 5–7 μm in diam. |
Note |
This species grows fast. When the petri dish is sealed with parafilm, it may lead to poor sporulation and the colony will remain pale yellow due to poor ventilation. |
Pathogenicity |
Unknown |
Specimens examined |
Taiwan, Hualien County, rice grains(cultivar Hualien 20), Jul 2018, Jie-Hao Ou, 18035 |
ITS |
CCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGGTCACGCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGAACCAACCAAACTCTTTCTGTAGTCCCCTCGCGGACGTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGATCGGCGTTGGGGATCGGGACCCCTCACACGGGTGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA |
EF1a |
GACTGAAGTGTCCAAAACATACCAATGACGGTGACATAGTACTTGGGAGTCTCGAACTTCCAGAGGGCAATGTCGATGGTGATACCACGCTCACGCTCGGCCTTGAGCTTGTCAAGAACCCACGCATACTTGAAGGAACCCTTGCCGAGTTCGGCGGCTTCCTATTGATTGAAGCATGATCAGCATAATGAACAAGAGATATTGAAAATAGACGATGCGATTGAACAAAAGCATGTGGTGGATGATGGAAAGAGGTACAGTGGCGATAGTGGGGTTGCCGTCAAACAAAATTGACACCCCACTAAAAGCCAAACAAGGCAGCGAGAAAATTTTTTGCTGTGCCAAAGGAGGGGTAGGTAGGCTGGCAAAGCGGGGTGATGCTGAAAAAAGAAAGACAAAACTGAGAACAGAATTGTCGGGCATATAATTGTGCCAAAAATTGATGGGAAAAGCAGTGAAATTAGCTTACCTTCTCGAACTTCTCGATGGTACGCTTGTCAATACCACCGCACTGGTAGATCAAGTGACCAGTCTATTAGGAAAAACGTTAGCTGGATGCTTCCATAATCAAGTTGCCCCGCGCCACCCGACAGAGTCGAGAGC |