Trichoderma asperelloides

Authors Samuels 2010
Strain 18035
Classification Hypocreales, Hypocreaceae, Trichoderma
Culture collection processing
Detection frequency Low
Figure Fig. 1 5-day-old colony on PDA Fig. 2 Trichoderma asperelloides. a. Sporulation on OA. b–d. Conidiophores, conidiogenous cells and conidia. e. Conidia. f. Chlamydospore (Bars= 5 μm, unless otherwise specified)
Colonies Colonies on PDA attaining >90-mm in 5 days at 25 °C, raised, cottony, pale yellow, with sparse radial furrows, producing brown, sticky exudate and diffuse yellow pigment with age. Dark green, slimy conidial mass produced on the surface after 7 days.
Conidiophores Conidiophores macronematous, hyaline, erect, with extensively, sometimes verticillately rebranching at right angle, slightly swollen at the branching points, 3.5–5 µm wide near the base of main axis.
Conidiogenous cells Conidiogenous cells phialidic, hyaline, lageniform, laterally or terminally, solitary or in whorls of 2–5, 7–15 × 2.5–3.5 µm.
Conidia Conidia globose to obovoid, slightly pigmented, dark green in mass, 3.0–4.0(–4.5) µm.
Chlamydospores Chlamydospores abundant on OA after 7 days, globose to subglobose, borne terminally, solitary, 5–7 μm in diam.
Note This species grows fast. When the petri dish is sealed with parafilm, it may lead to poor sporulation and the colony will remain pale yellow due to poor ventilation.
Pathogenicity Unknown
Specimens examined Taiwan, Hualien County, rice grains(cultivar Hualien 20), Jul 2018, Jie-Hao Ou, 18035
ITS CCGAGTTTACAACTCCCAAACCCAATGTGAACGTTACCAAACTGTTGCCTCGGCGGGGTCACGCCCCGGGTGCGTCGCAGCCCCGGAACCAGGCGCCCGCCGGAGGAACCAACCAAACTCTTTCTGTAGTCCCCTCGCGGACGTATTTCTTACAGCTCTGAGCAAAAATTCAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCCCTCCGGGGGATCGGCGTTGGGGATCGGGACCCCTCACACGGGTGCCGGCCCCGAAATACAGTGGCGGTCTCGCCGCAGCCTCTCCTGCGCAGTAGTTTGCACAACTCGCACCGGGAGCGCGGCGCGTCCACGTCCGTAAAACACCCAACTTTCTGAAATGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
EF1a GACTGAAGTGTCCAAAACATACCAATGACGGTGACATAGTACTTGGGAGTCTCGAACTTCCAGAGGGCAATGTCGATGGTGATACCACGCTCACGCTCGGCCTTGAGCTTGTCAAGAACCCACGCATACTTGAAGGAACCCTTGCCGAGTTCGGCGGCTTCCTATTGATTGAAGCATGATCAGCATAATGAACAAGAGATATTGAAAATAGACGATGCGATTGAACAAAAGCATGTGGTGGATGATGGAAAGAGGTACAGTGGCGATAGTGGGGTTGCCGTCAAACAAAATTGACACCCCACTAAAAGCCAAACAAGGCAGCGAGAAAATTTTTTGCTGTGCCAAAGGAGGGGTAGGTAGGCTGGCAAAGCGGGGTGATGCTGAAAAAAGAAAGACAAAACTGAGAACAGAATTGTCGGGCATATAATTGTGCCAAAAATTGATGGGAAAAGCAGTGAAATTAGCTTACCTTCTCGAACTTCTCGATGGTACGCTTGTCAATACCACCGCACTGGTAGATCAAGTGACCAGTCTATTAGGAAAAACGTTAGCTGGATGCTTCCATAATCAAGTTGCCCCGCGCCACCCGACAGAGTCGAGAGC