Authors | (Ganguly) B.L. Jain 1976 |
---|---|
Strain | 12001 |
Classification | Pleosporales, Pleosporaceae, Trichoconiella |
Culture collection | BCRC FU31231 |
Detection frequency | High |
Figure | Fig. 1 5-day-old colony on PDA Fig. 2 Trichoconiella padwickii. a, b. Conidia. c. Conidiophore. d. Conidiogenous cell. e. Sclerotium (Bars= 5 μm, unless otherwise specified)
Fig. 3 a. Sclerotia on the surface of brown rice. b. Sclerotia produced in culture. |
Colonies | Colonies on PDA attaining 28-mm in 5 days at 25 °C, azonated, fluffy, margins even, initially white, turning grey with age, reverse dark olivaceous brown with white margin. |
Conidiophores | Conidiophores macronematous, simple, rarely branched, subhyaline to light brown, multiseptate, up to 200 µm in length. |
Conidiogenous cells | Conidiogenous cells integrate, terminal, slightly swollen, bearing single conidium. |
Conidia | Conidia fusiform to obclavate, subhyaline to light brown, smooth to slightly echinulate, body with (3–)4–5 septa, slightly constricted at the septa, subtruncate at slightly thickened hilum, 45–70 × 12–22 µm, beaks present in most conidia, hyaline, with 1–5 septa, 50–120 × 2.5–3.5 µm. |
Sclerotia | Sclerotia produced abundantly when keep in complete dark or in old culture, globose, mostly submerge, wall reticulate, 70–100 µm in diameter. |
Note | With few exceptions, this species is mostly associated with rice. Morphologically this species is liable to be misidentified as Alternaria, and accordingly has been named under Alternaria. This species has poor sporulation in culture. To induce sporulation, refer to the note of Pyricularia oryzae.
Sclerotia can be induced under nitrogen limited and complete darkness condition. |
Pathogenicity | This species causes leaf spot (stackburn disease) and grain discoloration on rice. The detection rate on rice seeds can often be very high, up to 100% (Mew & Misra, 1994). |
Specimens examined | Taiwan, Chiayi County, rice grains (cultivar Tainan 11), Nov 2012, Jie-Hao Ou, 12052 Taiwan, Chiayi County, rice grains (cultivar Tainan 11), Jan 2013, Jie-Hao Ou, 13011 Taiwan, Ilan County, rice grains, Aug 2014, Jie-Hao Ou, 14105 Taiwan, Taitung County, rice grains (cultivar Taitung 30), Sep 2014, Jie-Hao Ou, 14112 Taiwan, Taitung County, rice grains (cultivar Taiken 2), Sep 2014, Jie-Hao Ou, 14126 Taiwan, Taitung County, rice grains (cultivar Kaohsiung 139), Nov 2014, Jie-Hao Ou, 14153 Taiwan, Taichung City, rice grains (cultivar Taichung 194), Dec 2014, Jie-Hao Ou, 14168 Taiwan, Ilan County, rice grains (cultivar Kaohsiung 147), Jan 2017, Jie-Hao Ou, 17003 Taiwan, Tainan City, rice grains (cultivar Tainan 11), Jun 2012, Jie-Hao Ou, 12001 |
ITS | AGGATCATTACACATATAAACTAGATCTCAGGGGAGGTTCGCCTCTTTCTGAGATGTATGTTATACCCATGTCTTTTGCGTACTACTTGTTTCCTTGGTAGGCTTGCCTGCCAATAGGACAAAATCAAAACCTTTTGTAATTGCAGTCAGCGTCAGAAATAACTTTTAATAGTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCATGGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTTTGCTTGGTGTTGGGCGTTTGTCTCGCGTTGCGCGTAGACTCGCCTTAAAGCGATTGGCAGCCGGCATATTGGCCTTGGAGCGCAGCACATTTTGCGAATCCAGTCATTTATGTTGGCGTCCATTAAGCCTATACATTTTGCTCTTGACCTC |