Tilletia horrida

Authors	Takah. 1896
Strain	17069
Classification	Tilletiales, Tilletiaceae, Tilletia
Culture collection	BCRC MU30528
Detection frequency	Low
Accession number	LC494385
Figure	Fig. 1 5-day-old colony on PDA Fig. 2 Tilletia horrida. a. Germinated teliospore and basidia. b. Basidiospores. c–f. Teliospores and tuberculate ornamentation (d, e). (Bars= 5 μm, unless otherwise specified) Fig. 3 Rice kernel smut caused by Tilletia horrida.
Colonies	Colonies on PDA attaining 10-mm in 5 days at 25 °C, margin irregular, white to light grey, reverse grey, often with reddish diffusing pigment.
Teliospores	Teliospores globose or subglobose, dark brown, tuberculate, (17–)20–25(–30) μm in diam. Teliospores germinated with in 24 hours at 25 °C on WA.
Basidia	Basidia (promycelia) simple, hyaline, up to 200 μm long, bearing 30–50 basidiospores terminally.
Basidiospores	Basidiospores filiform, straight or curved, hyaline, 30–40 × 1–2 µm.
Note	This species was found on fresh organically cultivated rice grain.
Pathogenicity	This species has been reported to cause rice kernel smut (Ou, 1985). It has been suggested that T. horrida is a synonym of T. barclayana which infect Panicoideae (Tullis & Johnson, 1952). However, it was subsequently identified as a distinct species based on its host range and molecular data (Castlebury et al., 2005; Pimentel et al., 1998).
Specimens examined	Taiwan, Ilan County, rice grains, Nov 2017, Jie-Hao Ou, 17069
ITS	ATTTAGTGATCCTGTCCGGATTGGCGCTAAGTCGTTGATACTTTCGAGTGTCAACGACTTGGTTCCTATTTCAACTCTTTACAAGCATAGAATGATAAAACATTGTCTTCGGACAGAAACAAnATACAACTTTTGACAACGGATCTCTTGGTTCTCCCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCCCTTGGGTATTCTCAAGGGCATGCCTGTTTGAGTGTCATAACAATCTCAACTCCCAGTTTTTTTGAAAGAGAAGACTGCTTGGAGTTGGTGATGGGCGTTTGCCAGACTTCAGTCTTGCTCGCCTGAAATATATTAGTGGATCTCTTTGGGTCGGTCTGACTATGTGTGATAATTTGATCGCATAGGATGTGCTCTGCGCAACCGGCTTTACATTGGAGACTTTCTGCTTCTAACACGGAATGGCTAGAGAGTAATTTTCTGCCATCGTTAGCTATATTGTCTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAA