Pseudopestalotiopsis cf. myanmarina

Strain 14149
Classification Amphisphaeriales, Sporocadaceae, Pseudopestalotiopsis
Culture collection BCRC FU31415
Detection frequency Low
Figure Fig. 1 5-day-old colony on PDA Fig. 2 Pseudopestalotiopsis cf. myanmarina. a, d. Conidiogenous cells and conidia. b, c. Conidia (Bars= 5 μm, unless otherwise specified)
Colonies Colonies on PDA attaining 66-mm at 25 °C after 5 days, sparse cottony, slightly zonate, white, producing scattered, black, conidiomata after 2 wks, reverse white, turning pale brown with age.
Conidiogenous cells Conidiogenous cells, hyaline, cylindrical or flask-shaped, 11-18 x 2-3 µm.
Conidia Conidia fusiform, mostly 4-septate, pale brown to brown except the subhyaline end cells, with three median cells concolorous, (23–)24–27 × 5.0–7.0 µm (avg. 25.9 × 6.1 µm), bearing 2–3(–4) apical appendages (2 appendages:3 appendages= 5:37), 23–30 µm (avg. 26.6 µm) long, and 0–1 basal appendages, if present, 2–10 µm (avg. 5.1 µm).
Note Strain 14149 share 100% identity in ITS and EF region with the ex-type strain of Pseudopestalotiopsis myanmarina (NBRC 112264, LC114025, LC114065). However, strain 14149 has distinctly smaller conidia ((23–)24–27 × 5.0–7.0 µm (avg. 25.9 × 6.1 µm)) than Pseudopestalotiopsis myanmarina strain NBRC 112264 (31–38.5 × 6.5–9 µm (avg. 35.9 × 8.1 µm), (Nozawa et al., 2017)).
Pathogenicity Unknown
Specimens examined Taiwan, Taitung County, rice grains (cultivar Kaohsiung 139), Oct 2014, Jie-Hao Ou, 14149
ITS AACTCCCAACCCATGTGAACTTACCTTTTGTTGCCTCGGCAGAGGTTACCTGGTACCTGGAGACAGGTTACCCTGTAGCAACTGCCGGTGGACTACTAAACTCTTGTTATTTTATGTAATCTGAGCGTCTTATTTTAATAAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCCTAGCTTAGTGTTGGGAATTTACAGTTATGTAATTCCTGAAATACAACGGCGGATCTGTGGTATCCTCTGAGCGTAGTAAATTATTTCTCGCTTTTGTTAGGTGCTGCAGCTCCCAGCCGCTAAACCCCCAATTTTTTGTGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
EF1a TACCATCGAGAAGTTCGAGAAGGTTAGTCATCTACTGATTTCCATCAACCGTCCTCAAATCTCATCAACATCTGCGTCATGATCTTCAACTTGCCAGTTGAAAATTATTTTTCGCACTTCCCACATTTTTCGACACTTACCCCGCCGCGAGGCACCCGCACGACCCCGCGGTGCAAACGAAAAATTTCTTATCACAGCCCCACTATGCACCAGCAACCATGCACATCTGATGAGACCCACTTTGAATTATTGCTAATACCTCCCAATAGGAAGCCGCCGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATCGATATCGCTCTCTGGAAGTTCGAGACCAACGAGTACAATGTCACCGTCATTGGTTAGTAGTCCCTGCCCTTGCCAAGCATCTTTGACATGACTCTTGACTAACATGGCCATACAGACGCTCCCGGTCACCGTGATTTCATCAAGAACATGATTACTGGTACCTCGCAGGCTGACTGCGCTATCCTCATCATTGCCGCTGGTACTGGTGAGTTCGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAGCACGCTCTGCTCGCTTTCACCCTCGGTGTCAAGCAGCTCATCGTTGCCATCAACAAGATGGACACTGCCAAGTGGTCCGAGGCCCGTTACAAGGAGATCATCAAGGAGACCTCCAACTTCATCAAGAAGGTTGGCTACAACCCCAAGGAGGT