Phaeotrichoconis crotalariae
| Authors |
(M.A. Salam & P.N. Rao) Subram. 1956 |
| Strain |
13007 |
| Classification |
Ascomycota, Phaeotrichoconis |
| Culture collection |
BCRC FU31260 |
| Detection frequency |
Low |
| Figure |
 Fig. 1 5-day-old colony on PDA  Fig. 2 Phaeotrichoconis crotalariae. a–d. Conidiophores and conidia. e. Microsclerotia. f. Germinated conidia. (Bars= 5 μm, unless otherwise specified)  Fig. 3 Maximum-likelihood (ML) tree obtained from aligned LSU sequence of the Phaeotrichoconis crotalariae 13007 and members of Pleosporaceae (Korsub et al., 2006). |
| Colonies |
Colonies on PDA attaining 68-mm at 25 °C after 5 days, cottony, pale orange, margin entire, reverse orange with pale brown margin. |
| Stromata |
Cylindrical stromata occasionally present. |
| Conidiophores |
Conidiophores macronematous, brown, straight or slightly curved, septate, simple, percurrent proliferation often observed, bearing 1–4 conidia, up to 400 µm long, 4–7 µm wide. |
| Conidiogenous cells |
Conidiogenous cells integrated, terminal or intercalary, geniculate, sympodial, with thickened conidial scars. |
| Conidia |
Conidia initially grey, turning dark brown with age, with both ends paler, straight, obclavate or ellipsoid, often with hyaline beak and thickened, truncated hilum. Conidium body 6–7(–8)-septate, (45–)60–80 × 12–18 µm. Beak multiseptate, up to 180 µm long, 1.5–2.5 µm wide. Germ tubes developed from both ends, hyaline, irregular, repeatedly branched. |
| Chlamydospores |
Chlamydospores abundant on OA, WA, CMA, especially when incubated in complete darkness, globose, subglobose, cylindrical or irregular, intercalary or terminal, thick-walled, dark brown, 4.0–8.0 × 4.0–13.0 μm. |
| Sclerotia |
Microsclerotia present, forming by aggregation of chlamydospores. |
| Note |
This species was first reported to cause leaf spots on Crotalaria verrucosa, and was identified as Trichoconis crotalariae (Salam & Rao, PN, 1954). However, Trichoconis crotalariae has dark vegetative hyphae and conidia which are different from Trichoconis; therefore, Subramanian (1956) transferred it to a new genus, Phaeotrichoconis. |
| Pathogenicity |
This species has been found on rice seeds and been associated with grain discoloration (S. Kumaraswamy, 1979; Vaidehi, 1971). |
| Specimens examined |
Taiwan, Tainan City, rice grains (cultivar Tainan 11), Jan 2013, Jie-Hao Ou, 13007 |
| ITS |
GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATCATTACACAAAAAAATATGAAGGCTGTACGCAGCTGAATTATTTTTCACCCATGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCCCGCCACCAGGACCACACCATAAACCTTTTTTATGCAGTTGCAATCAGCGTCAGTAAAACAAGTAATTAATTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTCTGCTTGGTGTTGGGCGTTTTTGTTATCCTTTTTGGGGGGTAACTCGCCTTAAAACAATTGGCAGCCGGCCTACTGGTTTCGGAGCGCAGCACAATTTTGCGCTTGCAACCAGCAAAAGAGGTTGGCGATCCATCAAGTCTACTTTTCTCACTTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
| LSU |
AACGGCGAGTGAAGCGGCAACAGCTCAAAATTTGAAATCTGGCTCTTTCAGAGTCCGAGTTGTAATTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTACGTGGTCGCTAGCTATTGCCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGCCAGACTTGCTTGCAGTTGCTCATCCGGGCTTTTGCCCGGTGCACTCTTCTGCAGGCAGGCCAGCATCAGTTTGGGCGGTGGGATAAAGGTCTCTGTCACGTACCTCTCTTCGGGGAGGCCTTATAGGGGAGGCGACATACCACCAGCCTGGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCGTAATGGCTGTAAGCGGCCCGTCTTGAAACACGGACCAAGGAGTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAGTGAACGGAGGTGGGAACCCGCAAGGGCGCACCATCGACCGATCCTGAAGTTTACGGAAGGATTTGAGTAAGAGCATGGCTGTTGGGACCCGAAAGATGGTGAACTATGCTTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGT |
| GPD |
GGCCGCATCGTCTTCCGCAATGCGTAGGTGCCCTTGAATCCATCCCTCTATTTTCAATGTGTACCAGAGCTAACGGCAGCTCGCAGCATTGAGCACAACGACGTCGACATTGTCGCCGTAAACGACCCCTTCATCGAGCCCCACTACGCTGTAAGCATCCCCAACACACAAGGCTACAGCCAGAGTAATGCTTTGTATGATTTGCTTCATCTTGAGCGCTCTGGCATCAATCATTGGCACAGGAGCATAGGCTAACGTGTAACACAGGCATACATGCTCAAGTATGACAGCACACACGGCCAGTTCAAGGGTGACATCAAGGTCGACGGCAACAACCTGACCGTCAATGGCAAGACCATCCGCTTCCACATGGAGAAGGACCCCGCCAACATCCCATGGAACGAGACCGGCGCCTACTACGTCGTTGAGTCCACCGGTGTCTTCACCACCACCGAGAAGGCCAAGGCTCACTTGAAGGGCGGAGCCAAGAAGGTTGTCATCTCTGCTCCCTCCGCTGACGCCCCCATGTTCGTCATGGGTGTCAACCACG |
| RPB2 |
TGGGGCCTGGTCTGCCCTGCTGATGGCCCGAAGGACAGGCCTGCGGTTAGGCCAAGATCCCGGATCTTTTGCGGAATGTCAGTGTTGGTAGCGATGCATCGCCCATTATCGACTTCATGACGCAACGCAACATGCAGCTTCTCGAGGAGTACGACCAGAATCAAAACCCGGATGCGACCAAGGTCTTCGTGAACGGTGGTTGGGTCGGTGTCCACTCCAATGCTCAACAATTGGTTACAGTTGTGCAGGAGCTTCGACGCAATGGAACTCTGTCTTACGAGATGAGTTTGATTCGTGATATTCGCGACAGAGAGTTCAAGATCTTCACAGATGCTGGTCGTGTCATGAGGCCTCTGTTCGTTGTGGAAAATGACATTCGAAAGCCGAACCGAAACCACCTTATCTTTACTAAGGAAATCAGTAATAAACTCAAGGCTGAACAACAGGAGACCAGTACAAGACAAGGCTGGAGTCAAGAGGAAGTTGAGCAGGCTACTTACGGCTGGAGAGGTCTTATTCAAGACGGTGTTGTCGAATACCTCGATGCCGAGGAAGAAGAAACTGCCATGATAACATTCTCTCCCGAGGATTTGGAAGAATGGCGAGAAATGAAGATGGGTTTGCCTGCAGCCGAGCGATCGACTGAGGGCAAAGATCGACTTCGCCGTCTCAAGCCCCTGCCGGATCCTCGTATCCACGCTTACACCCATTGCGAGATTCACCCGGCTATGATCTTGGGTATTTGCGCCAGTATCATTCCTTTC |