Phaeotrichoconis crotalariae

Authors (M.A. Salam & P.N. Rao) Subram. 1956
Strain 13007
Classification Ascomycota, Phaeotrichoconis
Culture collection BCRC FU31260
Detection frequency Low
Figure Fig. 1 5-day-old colony on PDA Fig. 2 Phaeotrichoconis crotalariae. a–d. Conidiophores and conidia. e. Microsclerotia. f. Germinated conidia. (Bars= 5 μm, unless otherwise specified) Fig. 3 Maximum-likelihood (ML) tree obtained from aligned LSU sequence of the Phaeotrichoconis crotalariae 13007 and members of Pleosporaceae (Korsub et al., 2006).
Colonies Colonies on PDA attaining 68-mm at 25 °C after 5 days, cottony, pale orange, margin entire, reverse orange with pale brown margin.
Stromata Cylindrical stromata occasionally present.
Conidiophores Conidiophores macronematous, brown, straight or slightly curved, septate, simple, percurrent proliferation often observed, bearing 1–4 conidia, up to 400 µm long, 4–7 µm wide.
Conidiogenous cells Conidiogenous cells integrated, terminal or intercalary, geniculate, sympodial, with thickened conidial scars.
Conidia Conidia initially grey, turning dark brown with age, with both ends paler, straight, obclavate or ellipsoid, often with hyaline beak and thickened, truncated hilum. Conidium body 6–7(–8)-septate, (45–)60–80 × 12–18 µm. Beak multiseptate, up to 180 µm long, 1.5–2.5 µm wide. Germ tubes developed from both ends, hyaline, irregular, repeatedly branched.
Chlamydospores Chlamydospores abundant on OA, WA, CMA, especially when incubated in complete darkness, globose, subglobose, cylindrical or irregular, intercalary or terminal, thick-walled, dark brown, 4.0–8.0 × 4.0–13.0 μm.
Sclerotia Microsclerotia present, forming by aggregation of chlamydospores.
Note This species was first reported to cause leaf spots on Crotalaria verrucosa, and was identified as Trichoconis crotalariae (Salam & Rao, PN, 1954). However, Trichoconis crotalariae has dark vegetative hyphae and conidia which are different from Trichoconis; therefore, Subramanian (1956) transferred it to a new genus, Phaeotrichoconis.
Pathogenicity This species has been found on rice seeds and been associated with grain discoloration (S. Kumaraswamy, 1979; Vaidehi, 1971).
Specimens examined Taiwan, Tainan City, rice grains (cultivar Tainan 11), Jan 2013, Jie-Hao Ou, 13007
ITS GGAAGTAAAAGTCGTAACAAGGTCTCCGTAGGTGAACCTGCGGAGGGATCATTACACAAAAAAATATGAAGGCTGTACGCAGCTGAATTATTTTTCACCCATGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCCCGCCACCAGGACCACACCATAAACCTTTTTTATGCAGTTGCAATCAGCGTCAGTAAAACAAGTAATTAATTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTCTGCTTGGTGTTGGGCGTTTTTGTTATCCTTTTTGGGGGGTAACTCGCCTTAAAACAATTGGCAGCCGGCCTACTGGTTTCGGAGCGCAGCACAATTTTGCGCTTGCAACCAGCAAAAGAGGTTGGCGATCCATCAAGTCTACTTTTCTCACTTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
LSU AACGGCGAGTGAAGCGGCAACAGCTCAAAATTTGAAATCTGGCTCTTTCAGAGTCCGAGTTGTAATTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTACGTGGTCGCTAGCTATTGCCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGCCAGACTTGCTTGCAGTTGCTCATCCGGGCTTTTGCCCGGTGCACTCTTCTGCAGGCAGGCCAGCATCAGTTTGGGCGGTGGGATAAAGGTCTCTGTCACGTACCTCTCTTCGGGGAGGCCTTATAGGGGAGGCGACATACCACCAGCCTGGACTGAGGTCCGCGCATCTGCTAGGATGCTGGCGTAATGGCTGTAAGCGGCCCGTCTTGAAACACGGACCAAGGAGTCTAACATCTATGCGAGTGTTTGGGTGTCAAGCCCGAGCGCGTAATGAAAGTGAACGGAGGTGGGAACCCGCAAGGGCGCACCATCGACCGATCCTGAAGTTTACGGAAGGATTTGAGTAAGAGCATGGCTGTTGGGACCCGAAAGATGGTGAACTATGCTTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGT
GPD GGCCGCATCGTCTTCCGCAATGCGTAGGTGCCCTTGAATCCATCCCTCTATTTTCAATGTGTACCAGAGCTAACGGCAGCTCGCAGCATTGAGCACAACGACGTCGACATTGTCGCCGTAAACGACCCCTTCATCGAGCCCCACTACGCTGTAAGCATCCCCAACACACAAGGCTACAGCCAGAGTAATGCTTTGTATGATTTGCTTCATCTTGAGCGCTCTGGCATCAATCATTGGCACAGGAGCATAGGCTAACGTGTAACACAGGCATACATGCTCAAGTATGACAGCACACACGGCCAGTTCAAGGGTGACATCAAGGTCGACGGCAACAACCTGACCGTCAATGGCAAGACCATCCGCTTCCACATGGAGAAGGACCCCGCCAACATCCCATGGAACGAGACCGGCGCCTACTACGTCGTTGAGTCCACCGGTGTCTTCACCACCACCGAGAAGGCCAAGGCTCACTTGAAGGGCGGAGCCAAGAAGGTTGTCATCTCTGCTCCCTCCGCTGACGCCCCCATGTTCGTCATGGGTGTCAACCACG
RPB2 TGGGGCCTGGTCTGCCCTGCTGATGGCCCGAAGGACAGGCCTGCGGTTAGGCCAAGATCCCGGATCTTTTGCGGAATGTCAGTGTTGGTAGCGATGCATCGCCCATTATCGACTTCATGACGCAACGCAACATGCAGCTTCTCGAGGAGTACGACCAGAATCAAAACCCGGATGCGACCAAGGTCTTCGTGAACGGTGGTTGGGTCGGTGTCCACTCCAATGCTCAACAATTGGTTACAGTTGTGCAGGAGCTTCGACGCAATGGAACTCTGTCTTACGAGATGAGTTTGATTCGTGATATTCGCGACAGAGAGTTCAAGATCTTCACAGATGCTGGTCGTGTCATGAGGCCTCTGTTCGTTGTGGAAAATGACATTCGAAAGCCGAACCGAAACCACCTTATCTTTACTAAGGAAATCAGTAATAAACTCAAGGCTGAACAACAGGAGACCAGTACAAGACAAGGCTGGAGTCAAGAGGAAGTTGAGCAGGCTACTTACGGCTGGAGAGGTCTTATTCAAGACGGTGTTGTCGAATACCTCGATGCCGAGGAAGAAGAAACTGCCATGATAACATTCTCTCCCGAGGATTTGGAAGAATGGCGAGAAATGAAGATGGGTTTGCCTGCAGCCGAGCGATCGACTGAGGGCAAAGATCGACTTCGCCGTCTCAAGCCCCTGCCGGATCCTCGTATCCACGCTTACACCCATTGCGAGATTCACCCGGCTATGATCTTGGGTATTTGCGCCAGTATCATTCCTTTC