| Authors | I. Miyake 1909 |
|---|---|
| Strain | 15035 |
| Classification | Pleosporales, Phaeosphaeriaceae, Phaeosphaeria |
| Culture collection | BCRC FU31216 |
| Detection frequency | Medium |
| Accession number | LC499620 |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Phaeosphaeria oryzae. a, b, d, e. Asci and ascospores. c. Perithecia on rice grain (Bars= 5 μm, unless otherwise specified) |
| Colonies | Colonies on PDA attaining 27-mm at 25 °C after 5 days, velvet, pale brown, slightly zonate, margin entire, reverse similar, pale brown. |
| Ascomata | Ascomata not observed in culture. Ascomata on rice hull globose, superficial or immersed, black. |
| Asci | Asci bitunicate, cylindrical, biseriate, 50–70 × 8–9 µm |
| Ascospores | Ascospores fusiform, slightly curved, golden brown, verruculose, 3-septate, slightly constricted at the septa, 16.5–20 × 3.5–5.0 µm (avg. 18.2 × 4.17 µm) |
| Note | This species is exclusively associated with rice, and may be the causal agent of discoloration of rice grains before harvest during. Strain 15035 shares 98.94% and 98.97% identities in ITS and RPB2 regions with the ex-type strain of Phaeosphaeria oryzae(CBS 110110; KF251186, KF296462) |
| Pathogenicity | Hara (1959) reported that this species causes leaf spot on rice. We found that it is often associated with grain discoloration, but the pathogenicity has yet to be proven by reinoculation. |
| Specimens examined | Taiwan, Changhua County, rice grains, May 2014, Jie-Hao Ou, 14061 Taiwan, Pingtung County, rice grains (cultivar Taiken 9), Aug 2014, Jie-Hao Ou, 14090 Taiwan, Taitung County, rice grains (cultivar Kaohsiung 139), Oct 2014, Jie-Hao Ou, 14108 Taiwan, Changhua County, rice grains (cultivar Kaohsiung 139), May 2015, Jie-Hao Ou, 15035 Taiwan, Ilan County, rice grains, Feb 2017, Jie-Hao Ou, 17034 |
| ITS | CCCAGCTACTTGTTTACACCCTTGTCTTTTTGCGTACTTATCGTTTCCTCGGCGGGCTTGCCTGCCGGTTGGACAACTTTATAACCTTTTTAAATCTTCAATCAGCGTCTGAATAATATACAATAATTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCATGGGGCATGCCTGTTCGAGCGTCATTTGTACCTTCAAGCTTTGCTTGGTGTTGGGTGCTTGTCTTTTTGTTAAGACTCACCTCAAAGTCATTGGCAGCCAGTGTTTTGGTAGTAAGCGCAGCACATTTTGCGTCTTGGTCCCTTAACAGCAGCATCCATCAAGCCATTTTCTCACTTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
| RPB2 | CTGACCGCTACACATATGCCTCAACTCTATCCCATCTGCGACGAACAAATACACCCGTCGGTCGTGACGGCAAGCTGGCGAAGCCTCGACAGCTTCACAACTCACATTGGGGACTTGTATGTCCCGCAGAAACACCTGAAGGACAGGCTTGCGGTCTCGTCAAGAATTTGTCTCTGATGTGCTACGTCAGTGTCGGCAGTGATGCCTCTCCTATCATCGACTTCATGTCGCAGCGCAATATGCAGCTGCTCGAGGAGTATGACCAAGTCATGAATCCCGAGGCTACCAAGGTCTTCGTCAATGGCGTCTGGGTGGGTGTTCATAATCAGGCACAGCAACTTGTGTCGGTTGTACAAGAGCTCAGGAGGAACGGAACCTTGTCCTACGAGATGAGTTTGATCCGTGACATTCGTGACCGCGAGTTCAAGATCTTCACAGACGCTGGTCGTGTCATGCGTCCTCTTTTCGTTGTCAACAATAATCCGTCGAGTGGCATGCAAAATCACCTCGTCTTTGACAGAAACATCAGCAATAAGCTGGTGGACGAGCAGGGTCATTCCGATGAGCGTGCAGGCTTGAGCGGAGAGGAGATTGCAGAACTGACATATGGTTGGAAGGGTCTCATCCAGGACGGTGTCATTGAATATCTGGACGCCGAAGAGGAGGAGACTGCCATGATCACCTTTTCCCCAGAAGACTTGGCTGAATGGCGAGAAATGAAGCATGGCGTGGCTTACGCTGAACGAGCTATGCTTGGCAAAGACCGACTCCGACGTATCAAGCCCAAGCCCGATCCGCGCGTTCATGCCTACACACATTGCGAGATTCACCCCGCCATGATTCTGGGTATCTGCGCTAGTATCATTCCTTTCCCTGATCATAACCAGTCGCCCCGTAACACTTACCA |