| Strain | 13020 |
|---|---|
| Classification | Amphisphaeriales, Sporocadaceae, Pestalotiopsis |
| Culture collection | BCRC FU30220 |
| Detection frequency | Low |
| Accession number | LC494382 |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Pestalotiopsis sp. 13020. a. Sporulation on PDA b–c. Conidia. d. Conidiogenous cells and conidia (Bars= 5 μm, unless otherwise specified) |
| Colonies | Colonies on PDA attaining 80-, >90-, 0-mm at 37 °C, 28 °C and 24 °C after 5 days, margins entire, velvety, white, producing scattered, black, conidiomata after 2 wks, reverse white to pale orange, slightly zonated. |
| Conidiogenous cells | Conidiogenous cells, hyaline, cylindrical, 10-25 x 1.5-2.5 µm. |
| Conidia | Conidia fusiform, mostly 4-septate, pale brown to brown except the subhyaline end cells, with the median and fourth cells slightly darker, 16.5–22 × 4.0–5.5 µm (avg. 19.5 × 4.9 µm), bearing 2–3(–4) apical appendages (2 appendages:3 appendages= 27:25), 9–19 µm (avg. 12.5 µm) long, and 1 basal appendages, 1–4 µm (avg. 3.18 µm). |
| Note | Strain 19010 is morphologically similar to Pestalotia kawakamii found on rice leaves by Sawada (1919). However, it has shorter apical appendages (5-10 µm).
Strain 19010 is phylogenetically closely related to Pestalotiopsis neolitseae, but can be distinguished from P. neolitseae by slightly narrower conidia (4.0–5.5 (avg. 4.9) vs. (4–)5–6 μm (avg. 6 μm)) |
| Pathogenicity | Unknown |
| Specimens examined | Taiwan, Chiayi County, rice grains (cultivar Tainan 11), Aug 2013, Jie-Hao Ou, 13020 |
| ITS | TAGAGTTTTCTAAACTCCCAACCCATGTGAACTTACCATTGTTGCCTCGGCAGAAGCTGCTCGGCGCGCCTTACCTTGGAACGGCCTACCCTGTAGCGCCTTACCCTGGAACGGCTTACCCTGCAACGGCTGCCGGTGGACTACCAAACTCTTGTTATTTTATGGTTATCTGAGCGTCTTATTTTAATAAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCCTAGCTTAGTGTTGGGAGCCTACTGCTTTTGCTAGCTGTAGCTCCTGAAATACAACGGCGGATCTGCGATATCCTCTGAGCGTAGTAATTTTTATCTCGCTTTTGACTGGAGTTGCAGCGTCTTTAGCCGCTAAACCCCCCAATTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA |
| LSU | CGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTCGGGTCCGAATTGTAATTTGTAGAGGATGATTTTGGTGCGGTATCTTCCGAGTTCCTTGGAACAGGACGCCTTAGAGGGTGAGAGCCCCGTACGGTTGAATGCCTAGCCTCTGTAAATCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGGGAAGGATTTGTGACCAGACTTTTTCTGGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTTGCCCAGTAAAGGCCAGCATCGATTTTCGGCGGCGGATAAAAGCAGTGGGAATGTGGCTCCCTACGGGGAGTGTTATAGCCCATTGTATAATACGCTGCTGGGGATCGAGGTACGCGCTTCTGCAAGGATGCTGGCGTAATGGTTATCAATCACCCGTCTTGAAACACGGACCAAGGAGTCGAACATTTATGCGAGTGTTTGGGTGTTAAACCCTCACGCGTAATGAAAGTGAACGGAGGTGAGAGCCCGTACGGGTGCATCATCGACCGATCCTGAAGTTTTCGGATGGATTTGAGTAAGAGCATAACTGTTCGGACCCGAAAGATGGTGAACTATGCGTGGATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGTCAAATCTGCGCATGGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTACCGCCGAAGTTTCCCTCAGGATAGCAGTGTTGTCTTCAGTTTTATGAGGTAAAGCGAATGATTAGGGACTCGGGGGCGCTTTTTAGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTTACTTAATTGAACGTGGGCATTCGAATGTACCAACACTAGTGGGGCCATTTTTGGGTAAGCAGAACTGGCGATGCGGGATGAACCGAACGCGGGGTTAAGGTGCCGGAGTGGACGCTCATCAGACACCACAAAAGGTGTTAGTACATCTTGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCTCAAGCGTCCCACCCATACCTCGCCCTCAGGGTAGAAATGATGCCCTGAGGAGTAGGCGGCCGTGG |
| EF1a | CCACCACTACCGGTCACTTGATCTACAAGTGCGGTGGTATCGACAAGCGTACCATCGAGAAGTTCGAGAAGGTTAGTCATCCTCGCAATCCCATCATCCCCATTCTCATACCTCGCAAACGTTTTCCAACCGGTGTCGAAAATCGGTTTTCGCACCTGCCCATTTTCCCAGACACTTACCCCGCCGCACGACCCCGCGGTGCAAACGAAAAATTTCTTATCACAGCCCCACTTCACACAACATTTTGGCAGCCACGCACTTTGCATGACCCACAGTGAACAATTGCTGACCCCGCCAAATAGGAAGCCGCCGAGCTCGGAAAGGGTTCCTTCAAGTACGCATGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATTGATATCGCTCTCTGGAAGTTCGAGACCAACGAGTACAATGTCACCGTCATTGGTTAGTATTCCTGTCCACAGAATGTATCATGTCTCCGAACTCAGACTAACATCACAATACAGACGCTCCCGGTCACCGTGATTTCATCAAGAACATGAT |