Authors | Maharachch., K.D. Hyde & Crous 2014 |
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Strain | 14064 |
Classification | Amphisphaeriales, Sporocadaceae, Pestalotiopsis |
Culture collection | BCRC FU31263 |
Detection frequency | Low |
Figure | Fig. 1 5-day-old colony on PDA Fig. 2 Pestalotiopsis oryzae. a. Sporulation on PDA b–c. Conidia. d. Conidiogenous cells and conidia (Bars= 5 μm, unless otherwise specified) |
Colonies | Colonies on PDA attaining 64-mm at 25 °C after 5 days, sparse cottony, slightly zonate, white, producing scattered, black, conidiomata after 2 wks, reverse white, turning pale brown with age. |
Conidiomata | Conidiomata pycnidial, composed of textura globulosa, aerial, superfical or submerged. |
Conidiogenous cells | Conidiogenous cells, hyaline, cylindrical or flask-shaped, 10-19 x 2-4 µm. |
Conidia | Conidia fusiform, mostly 4-septate, pale brown to brown except the subhyaline end cells, with the median and fourth cells slightly darker, 24–35 × 5.0–6.5 µm (avg. 28.5 × 5.7 µm), bearing 2–3(–4) apical appendages (2 appendages:3 appendages= 6:33), 8.5–18 µm (avg. 12.5 µm) long, and 0–1 basal appendages, if present, 1–7 µm (avg. 3.90 µm). |
Note | Strain 14064 shares 100% identity in ITS region with the ex-type strain of Pestalotiopsis oryzae (CBS 353.69, KM199496). According to the original description (Maharachchikumbura et al., 2014), CBS 353.69 has slightly wider conidia (6–8 µm(avg. 7 µm)).
Another species also isolated from rice, Pestalotia oryzae, has larger spores (33–35 × 7–10 µm) and longer appendages (20–45 µm) (Hara, 1918) |
Pathogenicity | Unknown |
Specimens examined | Taiwan, Changhua County, rice grains, May 2014, Jie-Hao Ou, 14064 |
ITS | TTATAGAGTTTTCTAAACTCCCAACCCATGTGAACTTACCATTGTTGCCTCGGCAGAAGCTGCTCGGTGCACCCTACCTTGGAACGGCCTACCCTGTAGCGCCTTACCCTGGAACGGCTTACCCTGTAACGGCTGCCGGTGGACTACCAAACTCTTGTTATTTTATTGTAATCTGAGCGTCTTATTTTAATAAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCCTAGCTTAGTGTTGGGAGCCTACTGCTTTTGCTAGCTGTAGCTCCTGAAATACAACGGCGGATCTGCGATATCCTCTGAGCGTAGTAAATTTTTATCTCGCTTTTGACTGGAGTTGCAGCGTCTTTGGCCGCTAAATCCCCCAATTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA |
LSU | GCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTCGGGTCCGAATTGTAATTTGTAGAGGATGATTTTGGTGCGGTATCTTCCGAGTTCCTTGGAACAGGACGCCTTAGAGGGTGAGAGCCCCGTACGGTTGAATGCCTAGCCTCTGTAAATCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGGGAAGGATTTGTGACCAGACTTTTTCTGGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTTGCCCAGTAAAGGCCAGCATCGATTTTCGGCGGCGGATAAAAGCAGTGGGAATGTGGCTCCCTACGGGGAGTGTTATAGCCCATTGTATAATACGCTGCTGGGGATCGAGGTACGCGCTTCTGCAAGGATGCCGGCCA |