Pestalotiopsis oryzae

Authors Maharachch., K.D. Hyde & Crous 2014
Strain 14064
Classification Amphisphaeriales, Sporocadaceae, Pestalotiopsis
Culture collection BCRC FU31263
Detection frequency Low
Figure Fig. 1 5-day-old colony on PDA Fig. 2 Pestalotiopsis oryzae. a. Sporulation on PDA b–c. Conidia. d. Conidiogenous cells and conidia (Bars= 5 μm, unless otherwise specified)
Colonies Colonies on PDA attaining 64-mm at 25 °C after 5 days, sparse cottony, slightly zonate, white, producing scattered, black, conidiomata after 2 wks, reverse white, turning pale brown with age.
Conidiomata Conidiomata pycnidial, composed of textura globulosa, aerial, superfical or submerged.
Conidiogenous cells Conidiogenous cells, hyaline, cylindrical or flask-shaped, 10-19 x 2-4 µm.
Conidia Conidia fusiform, mostly 4-septate, pale brown to brown except the subhyaline end cells, with the median and fourth cells slightly darker, 24–35 × 5.0–6.5 µm (avg. 28.5 × 5.7 µm), bearing 2–3(–4) apical appendages (2 appendages:3 appendages= 6:33), 8.5–18 µm (avg. 12.5 µm) long, and 0–1 basal appendages, if present, 1–7 µm (avg. 3.90 µm).
Note Strain 14064 shares 100% identity in ITS region with the ex-type strain of Pestalotiopsis oryzae (CBS 353.69, KM199496). According to the original description (Maharachchikumbura et al., 2014), CBS 353.69 has slightly wider conidia (6–8 µm(avg. 7 µm)).
Another species also isolated from rice, Pestalotia oryzae, has larger spores (33–35 × 7–10 µm) and longer appendages (20–45 µm) (Hara, 1918)
Pathogenicity Unknown
Specimens examined Taiwan, Changhua County, rice grains, May 2014, Jie-Hao Ou, 14064
ITS TTATAGAGTTTTCTAAACTCCCAACCCATGTGAACTTACCATTGTTGCCTCGGCAGAAGCTGCTCGGTGCACCCTACCTTGGAACGGCCTACCCTGTAGCGCCTTACCCTGGAACGGCTTACCCTGTAACGGCTGCCGGTGGACTACCAAACTCTTGTTATTTTATTGTAATCTGAGCGTCTTATTTTAATAAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCCTAGCTTAGTGTTGGGAGCCTACTGCTTTTGCTAGCTGTAGCTCCTGAAATACAACGGCGGATCTGCGATATCCTCTGAGCGTAGTAAATTTTTATCTCGCTTTTGACTGGAGTTGCAGCGTCTTTGGCCGCTAAATCCCCCAATTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA
LSU GCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCCCTCGGGTCCGAATTGTAATTTGTAGAGGATGATTTTGGTGCGGTATCTTCCGAGTTCCTTGGAACAGGACGCCTTAGAGGGTGAGAGCCCCGTACGGTTGAATGCCTAGCCTCTGTAAATCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTAAATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGGGAAGGATTTGTGACCAGACTTTTTCTGGGCGGATCATCCGGGGTTCTCTCCGGTGCACTTTGCCCAGTAAAGGCCAGCATCGATTTTCGGCGGCGGATAAAAGCAGTGGGAATGTGGCTCCCTACGGGGAGTGTTATAGCCCATTGTATAATACGCTGCTGGGGATCGAGGTACGCGCTTCTGCAAGGATGCCGGCCA