| Authors | F. Liu, L. Cai & Crous 2018 |
|---|---|
| Strain | 19010 |
| Classification | Amphisphaeriales, Sporocadaceae, Pestalotiopsis |
| Culture collection | processing |
| Detection frequency | Low |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Pestalotiopsis hispanica. a. Sporulation on PDA b. Conidiogenous cells and conidia. c–d. Conidia (Bars= 5 μm, unless otherwise specified) |
| Colonies | Colonies on PDA attaining 56-mm at 25 °C after 5 days, margin entire to filiform, cottony, white, producing scattered, black, conidiomata after 2 wks, reverse pale orange. |
| Conidiogenous cells | Conidiogenous cells, hyaline, cylindrical. |
| Conidia | Conidia fusiform, mostly 4-septate, pale brown to brown except the subhyaline end cells, with the median cell slightly darker, 20.5–28 × 5–7 µm (avg. 24.3 × 6.2 µm), bearing 2–4 apical appendages, 9–20 µm (avg. 14.8 µm) long, and 1-2 (mostly 1) basal appendages, 2–5.5 µm (avg. 3.96 µm). |
| Note | Strain 19010 is phylogenetically closely related to Pestalotiopsis brachiata and Pestalotiopsis hispanica. The ITS and EF1a sequence similarities between strain 19010 and P. hispanica CBS 115391 and P. brachiata LC2988 are 100%/99.0% and 99.37%/99.62%, respectively. Morphologically, 19010 and P. hispanica CBS 115391 and
P. brachiata LC2988 has a similar conidia size; the number of apical appendages is 2-4/2-4/1-4 µm, with a length 9–20/2–14/16–28.5 µm, and the number of basal appendages is 1-2/0-1/1-4, with a length of 2–5.5/1.5–4.5/5.5–9.5 µm. Strain 19010 is morphologically similar to Pestalotia kawakamii found on rice leaves by Sawada (1919). However, it has shorter apical appendages (5-10 µm) and narrower conidium body (4-5 µm). |
| Pathogenicity | Unknown |
| Specimens examined | Taiwan, Tainan City, rice grains, Jan 2020, Jie-Hao Ou, 19010 |
| ITS | GTAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTATAGAGTTTTCTAAACTCCCAACCCATGTGAACTTACCATTGTTGCCTCGGCAGAAGCTGCTCGGTACACCCTACCTTGGAACGGCCTACCCTGTAGCGCCTTACCCTGGAACGGCTTACCCTGTAACGGCTGCCGGTGGACTACCAAACTCTTGTTATTTTATTGTAATCTGAGCGTCTTATTTTAATAAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCATTAGTATTCTAGTGGGCATGCCTGTTCGAGCGTCATTTCAACCCTTAAGCCTAGCTTAGTGTTGGGAGCCTACTGCTTTTACTAGCTGTAGCTCCTGAAATACAACGGCGGATCTGCGATATCCTCTGAGCGTAGTAAATTTTTATCTCGCTTTTGACTGGAGTTGCAGCGTCTTTAGCCGCTAAATCCCCCAATTTTTAATGGTTGACCTCGGATCAGGTAGGAATACCCGCTGAACTTAA |
| EF1a | GCAAGTCCACCACTACCGGTCACTTGATCTACAAGTGCGGTGGTATCGACAAGCGTACCATCGAGAAGTTCGAGAAGGTTAGTCATCCTCGCAATCCCATCATCCTCATCATCATCATCATCACCACCTCGCAAACGTTGCCACACCGGTGCCGAAAATCTGGTTTTCGCACCTGCCCATTTTCCCAGACACTTACCCCGCCGCACGACCCCGCGGTGCAAACGAAAAATTTCTTATCATAGCCCCACATCACACAAACATTTTGGCAGCCACGCACTTTGCATGACCCACAATGAACAATTGCTGACCCCGCCAAATAGGAAGCCGCCGAGCTCGGAAAGGGTTCCTTCAAGTACGCATGGGTTCTTGACAAGCTCAAGGCCGAGCGTGAGCGTGGTATCACCATTGATATCGCTCTCTGGAAGTTCGAGACCAACGAGTACAATGTCACCGTCATTGGTTAGTATCCCTGTCCACAACATGTGTCATGTCTCCAAACTCAAGACTAACCTTGCAATACAGACGCTCCCGGTCACCGTGATTTCATCAAG |