| Authors | Y. Marín, Cheew. & Crous 2017 |
|---|---|
| Strain | 13058 |
| Classification | Pleosporales, Pleosporaceae, Curvularia |
| Culture collection | BCRC FU30223 |
| Detection frequency | Medium |
| Accession number | LC494368 |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Curvularia pseudobrachyspora. a–c, e. Conidiophores and conidia. d. Vegetative hyphae with brown exudates. f. Conidia (Bars= 5 μm, unless otherwise specified) |
| Colonies | Colonies on PDA attaining 68-, 64-, 5-mm at 24 °C, 28°C and 37 °C after 5 days, margins entire, floccose, reverse dark, often with orange diffusing pigment, forming dark black, cylindrical stromata after several weeks. |
| Conidiophores | Conidiophores macronematous, brown, up to 500 µm long. |
| Conidiogenous cells | Conidiogenous cells integrated, terminal or intercalary, geniculate, sympodial, with darkened and thickened conidial scars. |
| Conidia | Conidia straight or slightly curved, clavate, ellipsoid or fusiform, smooth, with inconspicious hilum, 2–4-septate(mostly 3) , 20–27 × 9–13 µm; first cell of mature conidia often slightly echinulate; third cell from base often larger and darker than the others. |
| Note | Curvularia pseudobrachyspora is morphologically close to C. brachyspora. C. pseudobrachyspora was proposed by Marin-Felix et al. (2017) to separate C. brachyspora based on their differences in ITS, GPD, and EF1a genes, with 99.78%, 98.61%, and 99.30% similarity respectively. C. pseudobrachyspora possessed shorter conidiophores (up to 420 μm), and was reported on (Eleusine indica) in Thailand. C. pseudobrachyspora is also closely related to C. protuberans. C. protuberans differs from C. pseudobrachyspora (type strain CPC 28808) only in 1 bp in EF1a (ITS and GPD are exactly the same). However, Raza et al. (2019) proposed C. protuberans as a new species by the smaller conidia (9.5–25.5 x 6–19.5 μm vs. 21.5–27 x 8–14 μm) and prominent hilum. As the morphological features used to characterize C. protuberans usually vary, this treatment should be reexamined. |
| Pathogenicity | Unknown |
| Specimens examined | Taiwan, Taichung City, rice grains (cultivar Tainan 11), Sep 2013, Jie-Hao Ou, 13058 Taiwan, Pingtung County, rice grains, Jan 2014, Jie-Hao Ou, 14029 Taiwan, Kaohsiung City, rice grains, Jan 2014, Jie-Hao Ou, 14030 |
| ITS | TAAAATATGAAGGCTTCGGCTGGATTATTTTTATCACCCTTGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCCCGCCACCAGGACCACACCATAAACCTTTTTTTATGCAGTTGCAATCAGCGTCAGTATAACAAATGTAAATCATTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTTTGCTTGGTGTTGGGCGTCTTTTGTCTTTGGCCTCGCCCAAAGACTCGCCTTAAAACGATTGGCAGCCGGCCTACTGGTTTCGCAGCGCAGCACATTTTTGCGCTTGCAATCAGCAAAAAGGACGGCAATCCATCAAGACTACATTTTTACGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
| EF1a | CCGCCGGTACTGGTGAGTTCGAGGCTGGTATCTCCAAGGATGGTCAGACTCGTGAGCACGCCCTGCTTGCCTACACCCTCGGTGTCAAGCAGCTCATCGTCGCCATCAACAAGATGGACACCACCAAGTGGTCTGAGGAGCGTTACCAGGAAATCATCAAGGAAACCTCCAACTTCATCAAGAAGGTCGGCTACAACCCCAAGCACGTTCCCTTCGTCCCCATCTCCGGTTTCAACGGAGACAACATGATTGAGGCTTCCACCAACTGCCCATGGTACAAGGGTTGGGAGAAGGAGACCAAGGCCAAGGCCACTGGTAAGACCCTCCTCGAGGCCATCGACGCCATCGACCCCCCTGTCCGTCCTACCGACAAGCCCCTCCGCCTTCCTCTCCAGGATGTCTACAAGATTGGCGGTATTGGCACGGTTCCCGTCGGTCGTGTCGAGACCGGTATCATCAAGCCCGGTATGGTCGTCACCTTCGCCCCCGCTGGTGTCACCACCGAAGTCAAGTCCGTCGAGATGCACCACGAGCAGCTTACTGAGGGTGTCCCCGGTGACAACGTCGGCTTCAACGTCAAGAACGTCTCCGTCAAGGAGATCCGTCGTGGTAACGTTGCCGGTGACTCCAAGAGCGACCCTCCCAAGGGCTGCGAGTCTTTCAACGCCCAGGTCATCGTCCTCAACCACCCTGGTCAGGTCGGTGCCGGTTACGCACCAGTCCTTGACTGCCACACTGCCCACATTGCCTGCAAGTTCTCCGAGCTCCTCGAGAAGATCGACCGCCGTACCGGAAAGTCTGTTGAGAACTCCCCCAAGTTCATCAAGTCCGGTGACGCTGCCATCGTCAAGATGGTTCCCTCTAAGCCCATG |
| GPD | CGTATCGTCTTCCGCAATGCGTAGGTGCCCTTGAATCCATTGATTCCGTGTATATCAATGCTAACGGTAGCCCGCAGCATCGAGCACAACGACGTCGAGATCGTCGCCGTAAACGACCCTTTCATTGAGCCCCACTACGCTGTAAGCATCCCCCAGTACAGACTCGTTTCGACGGTCAGAGCAATGGCTTGCATCACTTGCTTTGGCCCGACGCCTCGGCATCATTCATTGGTAGAGCAGTGCAGGCTAACATCTACATAGGCATACATGCTCAAGTATGACAGCACACACGGCCAGTTCAAGGGCGACATCAAGGTTGACGGCAACAACCTGACTGTCAACGGCAAGACTGTCCGCTTCCACATGGAGAAGGACCCCGCCAACATCCCATGGAGCGAGACCGGCGCCTACTACGTCGTTGAGTCCACCGGTGTCTTCACCACCACCGAGAAGGCCAAGGCTCACTTGAAGGGTGGAGCCAAGAAGGTTGTCATCTCTGCTCCTTCCGCCGATGCCCCCATGTTCGTCATGGGTGTCA |