| Authors | Y. Marín, Senwanna & Crous 2017 |
|---|---|
| Strain | 13012 |
| Classification | Pleosporales, Pleosporaceae, Curvularia |
| Culture collection | BCRC FU31455 |
| Detection frequency | Low |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Curvularia dactyloctenicola. a–b. Conidiophores and conidia. c Conidia. d. Chlamydospores in culture. e. Chlamydospores and conidiophores (Bars= 5 μm, unless otherwise specified)
Fig. 3 Maximum-likelihood (ML) tree obtained from aligned ITS, GPD sequences of the Curvularia dactyloctenicola and allied species. Type sequences are shown in bold. |
| Colonies | Colonies on PDA attaining 29-, 37-, 13-mm at 24 °C, 28°C and 37 °C after 5 days, margins entire, floccose, reverse initially white with diffused brown pigment, black, zonated when older. |
| Conidiophores | Conidiophores macronematous, brown, flexuous, septate, simple, up to 300 µm, 3–6 µm wide, sometimes arising from a germinating chlamydospore. |
| Conidiogenous cells | Conidiogenous cells integrated, terminal or intercalary, geniculate, sympodial, with darkened and thickened conidial scars. |
| Conidia | Conidia straight or curved, pale brown, nearly concolor, with slightly thickened hilum at the base, mostly 3-septate, the third cell often disproportionately swollen, 18–25 × 7–12 µm. |
| Chlamydospores | Chlamydospores thick-walled, globose, dark-brown, often found in aggregates of varying size. |
| Note | Cylindrical stromataprotruding from medium surface often present in old cultures, especially in the cultures incubated in complete darkness. Strain 13012 shares 99.8% and 100% identities in ITS and GPD regions with the ex-type strain of Curvularia dactyloctenicola (CPC 28810; MF490815, MF490837). Although both strains obtained in this study are phylogenetically closely related to Curvularia dactyloctenicola, There are slightly different in morphology. Strain 13012 produced abundant chlamydospores and microsclerotia in culture, which was not observed by Marin-Felix et al. (2017). In addition, the conidia were significantly wider, with the enlarged third cells being more pronounced. |
| Pathogenicity | Unknown. This species has not been previously found on rice, but has been reported to be associated with leaf spots on Dactyloctenium aegyptium. |
| Specimens examined | Taiwan, Tainan City, rice grains (cultivar Tainan 11), Dec 2012, Jie-Hao Ou, 13012 Taiwan, Tainan City, rice grains(cultivar Taichung Sen No. 10), May 2019, Yi-Chen, Li, 20001 |
| ITS | TGAAGGCTGTCCGCAGCTGGAGTATTTTATTACCCTTGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCTCGCCACCAGGACCACCAAATAAACCTTTTTTATGCAGTTGCAATCAGCGTCAGTACAAACAATGTAAATCATTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTTTGCTTGGTGTTGGGCGTTTTTGTCTTTGGTCGCCCAAAGACTCGCCTTAAAGTGATTGGCAGCCGGCCTTTCTGGTTTCGCAGCGCAGCACATTTTTGCGCTTGCCATCAGCAAGACGGCAATCCATCAAGCCTCCTTCTCACGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGC |
| GPD | CGTATCGTCTTCCGCAATGCGTAGGTGCCCTTGAATCCATTGATTCAGCGTGTATCGAAGCTAATCGAAGCTCGCAGCATCGAGCACAACGACGTCGAGATTGTCGCCGTGAACGACCCCTTCATCGAGCCCCACTACGCTGTAAGCATCCCCAGCACAGAATCCTTCCGTCAGAGCGATGCTTTGCATCATTGATTCCATCCTGGCATGATCCATTGGCGGAACAGTACAAGCTAACATGTCCATAGGCATACATGCTCAAGTATGACAGCACACACGGCCAGTTCAAGGGCGACATCAAGGTTGACGGCAACAACCTGACTGTCAACGGCAAGACCGTCCGCTTCCACATGGAGAAGGACCCCGCCAACATCCCATGGAGCGAGACCGGCGCTTACTACGTCGTTGAGTCCACTGGTGTCTTCACCACCACCGAGAAGGCCAAGGCTCACTTGAAGGGTGGAGCCAAGAAGGTTGTCATCTCTGCTCCCTCCGCCGATGCCCCTATGTTCGTCATGGGTGTCAACCACGA |