| Authors | Y.P. Tan & R.G. Shivas 2018 |
|---|---|
| Strain | 14051 |
| Classification | Pleosporales, Pleosporaceae, Curvularia |
| Culture collection | BCRC FU31234 |
| Detection frequency | Medium |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Curvularia beasleyi. a–c. Conidiophores and conidia. d, e. Conidia. f. Sporulation on OA (Bars= 5 μm, unless otherwise specified)
Fig. 3 Maximum-likelihood (ML) tree obtained from aligned ITS, GADPH sequences of the Curvularia beasleyi and allied species. |
| Colonies | Colonies on PDA attaining 56-mm at 25°C after 5 days, margins entire, raised, floccose, reverse dark olivaceous with yellow diffused pigment and white margins. |
| Conidiophores | Conidiophores macronematous, light brown, flexuous, septate, simple or rarely branched, percurrent proliferation occasionally observed, up to 100 µm, 3–5 µm wide. |
| Conidiogenous cells | Conidiogenous cells integrated, terminal or intercalary, geniculate, sympodial, with darkened and thickened conidial scars. |
| Conidia | Conidia straight, cylindrical, smooth, with slightly darkened and thickened hilum, brown, (1–)3–5 distoseptate (mostly 3–5). Ratio of 1-, 2-, 3-, 4-, 5-septate conidia=5:2:19:5:34. 1-septate conidia 7–13 × 5–8 µm; 2-septate conidia 10–15 × 6–9 µm; 3-septate conidia 16–20 × 7–9 µm; 4-septate conidia 18–26 × 8–10 µm; 5-septate conidia 20–29 × 7–10.5 µm. |
| Note | Curvularia beasleyi is morphologically similar to C. hawaiiensis, but can be distinguished by having narrower conidia with fewer septa. Strain 14051 shares 100% and 99.13% identities in ITS and GPD regions with the ex-type strain of C. beasleyi (BRIP10972; MH414892, MH433638). |
| Pathogenicity | Unknown |
| Specimens examined | Taiwan, Taichung City, rice grains, Mar 2014, Jie-Hao Ou, 14055 Taiwan, Tainan City, rice grains (cultivar Tainan 11), Mar 2014, Jie-Hao Ou, 14051 Taiwan, Pingtung County, rice grains (cultivar Taiken 9), Aug 2014, Jie-Hao Ou, 14091 Taiwan, Pingtung County, rice grains (cultivar Taiken 9), Aug 2014, Jie-Hao Ou, 14094 Taiwan, Miaoli County, rice grains (cultivar Miaoli 2), Dec 2014, Jie-Hao Ou, 14182 |
| ITS | CGAAGGCCGTTCGCGGCTGGACTATTTATTACCCTTGTCTTTTGCGCACTTGTTGTTTCCTGGGCGGGTTCGCTCGCCACCAGGACCACCATATAAACCTTTTTTATGCAGTTGCAATCAGCGTCAGTATAACAAATGTAAATCATTTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATACGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTTTGCTTGGTGTTGGGCGTTTTTGTCCCCCTAACAAGGGACTCGCCTTAAAAGGATTGGCAGCCGGCCTACTGGTTTCGCAGCGCAGCACATTTTTGCGCTTGCAATCAGCAAAAGAGGACGGCAATCCATCAAGACTCCTTCTCACGTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
| EF1a | TACCTCCCAGGCTGACTGCGCCATTCTCATCATTGCCGCCGGTACTGGTGAGTTCGAGGCTGGTATCTCCAAGGATGGTCAGACTCGTGAGCACGCTCTGCTCGCCTACACCCTCGGTGTCAAGCAGCTCATCGTCGCCATCAACAAGATGGACACCACCAAGTGGTCTGAGGAGCGTTACCAGGAAATCATCAAGGAGACCTCCAACTTCATCAAGAAGGTCGGCTACAACCCCAAGCACGTTCCCTTCGTCCCCATCTCCGGTTTCAACGGAGACAACATGATTGAGGCTTCCACCAACTGCCCCTGGTACAAGGGTTGGGAGAAGGAGACCAAGGCCAAGGCCACTGGTAAGACCCTCCTTGAGGCCATCGACGCCATCGACCCCCCTGTCCGTCCTACCGACAAGCCCCTCCGCCTTCCCCTCCAGGATGTCTACAAGATTGGTGGTATTGGCACGGTCCCCGTCGGTCGTGTCGAGACCGGTATCATCAAGCCCGGTATGGTCGTCACCTTCGCCCCCGCTGGTGTCACCACTGAAGTCAAGTCCGTCGAGATGCACCACGAGCAGCTCACCGAGGGTGTCCCCGGTGACAACGTCGGCTTCAACGTCAAGAACGTCTCCGTCAAGGAGATCCGTCGTGGTAACGTTGCCGGTGACTCCAAGAACGACCCCCCCAAGGGTTGCGAGTCCTTCAACGCCCAGGTCATCGTCCTCAACCACCCCGGTCAGGTCGGTGCCGGTTACGCACCAGTCCTTGACTGCCACACTGCCCACATTGCTTGCAAGTTCTCCGAGCTCCTCGAGAAGATCGACCGCCGTACCGG |
| RPB2 | TGGGGTCTTGTCTGCCCTGCTGAGACCCCCGAAGGACAGGCTTGCGGTCTGGTCAAGAACTTGTCTCTTATGTGTTATGTCAGTGTTGGTAGCGATGCATCGCCCATTATCGACTTCATGACACAACGTAACATGCAGCTTCTCGAAGAGTACGACCAGAACCAGAACCCGGATGCGACCAAGGTCTTCGTCAACGGTGTCTGGGTTGGTGTTCATTCAAATGCTCAGCAACTTGTCACGGTCGTGCAGGAGCTTCGCCGCAATGGAACTCTGTCTTACGAGATGAGTTTGATTCGTGACATTCGTGACAGAGAGTTCAAGATCTTCACAGATGCGGGGCGTGTCATGAGGCCTTTGTTCGTTGTAGAAAACGATATTCGAAAGCCAAACCGGAACCACCTCATCTTTACTAAGGAAATCAGCAACAAGCTCAAGGCTGAACAGCAAGAGACCAGTACAAGACAAGGCTGGAGTCAAGAGGAAGTCGAGCAGGCTACATACGGCTGGAGGGGTCTCATTCAAGACGGTGTCGTTGAATACCTCGATGCCGAGGAAGAAGAAACTGCAATGATAACATTCTCTCCCGAGGATTTGGAAGAATGGCGAGAAATGAAGATGGGTTTGCCTGCAGCCGAGCGATCAACTGAAGGCAAGGATCGACTTCGCCGTCTCAAGCCACTACCAGATCCTCGCATCCACGCCTACACCCATTGTGAGATTCACCCTGCTATGATTTTGGGTATTTGCGCCAGTATCATTCCCTTC |