Cladosporium tenuissimum

Authors	Cooke 1878
Strain	14084
Classification	Capnodiales, Cladosporiaceae, Cladosporium
Culture collection	BCRC FU31235
Detection frequency	Low
Figure	Fig. 1 5-day-old colony on PDA Fig. 2 Cladosporium tenuissimum. a. Intact conidiophores and conidia. b–c,e. Conidiophores and conidia. d. Sporulation on cornmeal agar. (Bars= 5 μm, unless otherwise specified)
Colonies	Colonies on PDA attaining 25-mm after 5 days at 25 °C, woolly, dark gray with white margin, reverse black, often with radial wrinkles.
Conidiophores	Conidiophores erect, solitary, dark brown to golden brown, multiseptate, occasionally branch, bearing up to 20 conidiogenous cells, geniculate or nodulose at conidiogenous loci, 2–3 μm in width, up to 2 mm long in old cultures.
Conidiogenous cells	Conidiogenous cells integrate, intercalary or terminal, often bearing 1–5 conidiogenous sites in whorl, slightly swollen.
Conidia	Conidia light brown to golden brown, catenate, mostly 2–4 in chain, ellipsoid, fusiform or obovoid, 2–5 × 2–3 μm. Secondary ramoconidia which give rise to 2–3 branches light brown to golden brown, cylinrical, with one end truncated, 5–10 × 2–3 μm.
Note	Sporulation was poor on nutrient-rich agar (e.g. PDA) for isolates of this species. Water agar or cornmeal agar are more suitable for proper sporulation.
Pathogenicity	This species is considered as saprophytic or endophytic and can often be isolated from healthy rice leaves (Fisher & Petrini, 1992).
Specimens examined	Taiwan, Tainan City, rice grains (cultivar Tainan 11), Jul 2014, Jie-Hao Ou, 14084
ITS	CTAACCACCGGGATGTTCATAACCCTTTGTTGTCCGACTCTGTTGCCTCCGGGGCGACCCTGCCTTCGGGCGGGGGCTCCGGGTGGACACTTCAAACTCTTGCGTAACTTTGCAGTCTGAGTAAATTTAATTAATAAATTAAAACTTTTAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTCGCTTGGTATTGGGCAACGCGGTCCGCCGCGTGCCTCAAATCGACCGGCTGGGTCTTCTGTCCCCTAAGCGTTGTGGAAACTATTCGCTAAAGGGTGCTCGGGAGGCTACGCCGTAAAACAAACCCATTTCTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA
EF1a	TGGCACGGACGACCATCACGACAAGACAGCATTGCCACTCAAAAACAACTTGCAGAGCACATCGCTGACAACGACAACAGGAAGCCGCCGAACTCGGCAAGGGTTCCTTCAAGTACGCATGGGTCCTCGACAAGCTGAAGTCCGAGCGTGAGCGTGGTATCACCATCGATATCGCCCTCTGGAAGTTCGAGACTCCCAAGTAAGCGCTTATCAGACACAACAAGCAAATGCACACTCGCTAACCTATTCACCCACCACAGGTACAACGTCACCGTCATTGACGCCCCCGGTCACCGTGATTTCATCAAGAACATGATCACTGGTACCTCCCAGGCTGACTGCGCCATCCTCATCATTGCCGCCGGTACTGGTGAGTTCGAGGCCGGTATCTCCAAGGACGGTCAGACCCGTGAGCACGCTCTTCTCGCCTACACCCTTGGTGTCAAGCAGCTCATCGTTGCCATCAACAAGATGGACACCACCAAGTGGTCTGAGGCCCGTTACCAGGAGATCATCAAGGAGACCTCCGGTTTCATCAAGAAGGTCGGCTTCAACCCCAAGCACGTTCCCTTCGTGCCCATCTCCGGTTTCAACGGTGACAACATGATCGACGTCTCCACCAACTGCCCCTGGTACAAGGGTTGGGAGAAGGAGACCAAGGCCAAGGCCACCGGCAAGACCCTCCTCGAGGCCATTGACGCCATCGACCCCCCCACCCGTCC