Cladosporium tenuissimum
Authors |
Cooke 1878 |
Strain |
14084 |
Classification |
Capnodiales, Cladosporiaceae, Cladosporium |
Culture collection |
BCRC FU31235 |
Detection frequency |
Low |
Figure |
Fig. 1 5-day-old colony on PDA Fig. 2 Cladosporium tenuissimum. a. Intact conidiophores and conidia. b–c,e. Conidiophores and conidia. d. Sporulation on cornmeal agar. (Bars= 5 μm, unless otherwise specified) |
Colonies |
Colonies on PDA attaining 25-mm after 5 days at 25 °C, woolly, dark gray with white margin, reverse black, often with radial wrinkles. |
Conidiophores |
Conidiophores erect, solitary, dark brown to golden brown, multiseptate, occasionally branch, bearing up to 20 conidiogenous cells, geniculate or nodulose at conidiogenous loci, 2–3 μm in width, up to 2 mm long in old cultures. |
Conidiogenous cells |
Conidiogenous cells integrate, intercalary or terminal, often bearing 1–5 conidiogenous sites in whorl, slightly swollen. |
Conidia |
Conidia light brown to golden brown, catenate, mostly 2–4 in chain, ellipsoid, fusiform or obovoid, 2–5 × 2–3 μm. Secondary ramoconidia which give rise to 2–3 branches light brown to golden brown, cylinrical, with one end truncated, 5–10 × 2–3 μm. |
Note |
Sporulation was poor on nutrient-rich agar (e.g. PDA) for isolates of this species. Water agar or cornmeal agar are more suitable for proper sporulation. |
Pathogenicity |
This species is considered as saprophytic or endophytic and can often be isolated from healthy rice leaves (Fisher & Petrini, 1992). |
Specimens examined |
Taiwan, Tainan City, rice grains (cultivar Tainan 11), Jul 2014, Jie-Hao Ou, 14084 |
ITS |
CTAACCACCGGGATGTTCATAACCCTTTGTTGTCCGACTCTGTTGCCTCCGGGGCGACCCTGCCTTCGGGCGGGGGCTCCGGGTGGACACTTCAAACTCTTGCGTAACTTTGCAGTCTGAGTAAATTTAATTAATAAATTAAAACTTTTAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTCGCTTGGTATTGGGCAACGCGGTCCGCCGCGTGCCTCAAATCGACCGGCTGGGTCTTCTGTCCCCTAAGCGTTGTGGAAACTATTCGCTAAAGGGTGCTCGGGAGGCTACGCCGTAAAACAAACCCATTTCTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
EF1a |
TGGCACGGACGACCATCACGACAAGACAGCATTGCCACTCAAAAACAACTTGCAGAGCACATCGCTGACAACGACAACAGGAAGCCGCCGAACTCGGCAAGGGTTCCTTCAAGTACGCATGGGTCCTCGACAAGCTGAAGTCCGAGCGTGAGCGTGGTATCACCATCGATATCGCCCTCTGGAAGTTCGAGACTCCCAAGTAAGCGCTTATCAGACACAACAAGCAAATGCACACTCGCTAACCTATTCACCCACCACAGGTACAACGTCACCGTCATTGACGCCCCCGGTCACCGTGATTTCATCAAGAACATGATCACTGGTACCTCCCAGGCTGACTGCGCCATCCTCATCATTGCCGCCGGTACTGGTGAGTTCGAGGCCGGTATCTCCAAGGACGGTCAGACCCGTGAGCACGCTCTTCTCGCCTACACCCTTGGTGTCAAGCAGCTCATCGTTGCCATCAACAAGATGGACACCACCAAGTGGTCTGAGGCCCGTTACCAGGAGATCATCAAGGAGACCTCCGGTTTCATCAAGAAGGTCGGCTTCAACCCCAAGCACGTTCCCTTCGTGCCCATCTCCGGTTTCAACGGTGACAACATGATCGACGTCTCCACCAACTGCCCCTGGTACAAGGGTTGGGAGAAGGAGACCAAGGCCAAGGCCACCGGCAAGACCCTCCTCGAGGCCATTGACGCCATCGACCCCCCCACCCGTCC |