| Strain | 15018 |
|---|---|
| Classification | Pleosporales, Pleosporaceae, Alternaria |
| Culture collection | processing |
| Detection frequency | Low |
| Figure |  Fig. 1 5-day-old colony on PDA  Fig. 2 Alternaria sp. 15018. a. Sporulation on PDA b–e. Conidiophores, secondary conidiophores (e) and conidia. f. Conidia. (Bars= 5 μm, unless otherwise specified)
Fig. 3 Maximum-likelihood (ML) tree obtained from aligned EF, GPDH, RPB2 sequences of the Alternaria sp. 15018 and allied species. |
| Colonies | Colonies on PDA attaining 33-mm at 25 °C after 5 days, margins entire, cottony, olivaceous brown with white margin; reverse dark olivaceous, zonated, paler towards the margin. |
| Conidiophores | Conidiophores erect, arising from submerged or aerial hyphae, brown, simple or with a lateral branch, bearing 1–3 conidiogenous loci, up to 200 µm long, 3–4 µm wide. Secondary conidiophores often observed, arising apically or laterally from conidium body, occasionally branched, up to 120 µm long, bearing 1–2 conidiogenous loci. |
| Conidiogenous cells | Conidiogenous cells tretic, integrated, terminal, sympodial, with thickened scars. |
| Conidia | Conidia brown, in chains, smooth or echinulate, mostly obclavate to obovoid, with (2–)3–7 slightly constricted transverse septa, 17–36 × 7–11 µm; conidia near the end of chains often slightly shorter and with fewer septa; the first four transverse sectors sometimes with 1–2 longitudinal or oblique septa; beak present on all conidia except the terminal conidia, 5–22 µm long. Primary conidial chains often comprised of 2–6 conidia, occasionally with 1 lateral seconday conidial chian. Secondary conidial chians short, comprised of 1–3 conidia. Tertiary conidial chains not observed. |
| Note | Strain 15018 forms a sister group with A. tomato and A. burnsii . In the four regions of ITS, EF, GPD, and RPB2, this strain shows 99.8%, 100%, 99.31%, 99.73% identities respectively with A. burnsii CBS 107.38, and 99.80%, 99.50%, 99.30%, 99.34% identities respectively with A. tomato CBS 103.30.
Alternaria sp. 15018 can be distinguished from Alternaria 14110 clade by having more transverse septa and fewer longitudinal septa. |
| Pathogenicity | Unknown |
| Specimens examined | Taiwan, Taichung City, rice grains (cultivar Taichung 65), Feb 2015, Jie-Hao Ou, 15018 |
| ITS | ATGAAGGCGGGCTGGAATCTCTCGGGGTTACAGCCTTGCTGAATTATTCACCCTTGTCTTTTGCGTACTTCTTGTTTCCTTGGTGGGTTCGCCCACCACTAGGACAAACATAAACCTTTTGTAATTGCAATCAGCGTCAGTAACAAATTAATAATTACAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAGTGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTCGAGCGTCATTTGTACCCTCAAGCTTTGCTTGGTGTTGGGCGTCTTGTCTCTAGCTTTGCTGGAGACTCGCCTTAAAGTAATTGGCAGCCGGCCTACTGGTTTCGGAGCGCAGCACAAGTCGCACTCTCTATCAGCAAAAGGTCTAGCATCCATTAAGCCTTTTTTCAACTTTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAA |
| LSU | GGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCTCTTTTAGAGTCCGAGTTGTAATTTGCAGAGGGCGCTTTGGCTTTGGCAGCGGTCCAAGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTACGTGGTCGCTGGCTATTGCCGTGTAAAGCCCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAATGGGAGGTACATTTCTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCAGCCAGACTTGCTTACAGTTGCTCATCCGGGTTCTTACCCGGTGCACTCTTCTGTAGGCAGGCCAGCATCAGTTTGGCCGGTAGGATCTTGGTCTTTTTCAGGAACCTAGGATGGAACACGCACCAAGGAGAGTCTTGGTCATTTAGAGGAAGT |
| EF1a | TTGATTCCGGCAAGTCCACCACCACCGGCCGTAAGCCCTCCCTCTCCGAACGACTGCGGGGTCAAACAACTTACACTGGGATACAGACTTGATCTACAAGTGCGGTGGTATCGACAAGCGTACCATCGAGAAGTTCGAGAAGGTATGGCATCACTTTTCTTTCACGCGGCCTGTTGCGCCCACCCGGTGCTTTCTCTGAGCGCGTAGCCAAAGCCTGGCTTATCGCGATGAGGGGCATTTTTGGGTGGTGGGGATGTGCGAACTTTTACGCGCTAGCGCTAGTCCGCATGCGGCCTTCGCGAACTCCAACGCCATGACGCACATGTAATTTCCCCATTCTGGCCACAGCGAGCTAACAAGTCTCACAGGAAGCCGCCGAACTCGGTAAGGGTTCCTTCAAGTACGCATGGGTCCTCGACAAGTTGAAGGCCGAGCGTGAGCGTGGTATCACCATCGACATTGCTCTCTGGAAGTTCGAGACTCCCAAGGTTAGTACCCTCTGCCTACTACATCAAGTTCTTTACAATGCTAACATGCTGTACTCAGTACTAT |
| GPD | TCGTCTTCCGCAATGCGTAAGTTTCGCCCAACTCGTCGATACAATCATCAAAGCTGACCGCATGCCACAGTATCGAGCACAACGACGTCGACATCGTCGCCGTAAACGACCCTTTCATCGAGCCCCACTACGCTGTAAGCTTCCCCAAGCACCTACACTACAGCCGCGGCCATCCAAATTGCGAAAACAGTTCTTGCGATGCGCTAGAGCTCCTGTGGTCGCAGAATGCAGGCTAACACATTCAGGCCTACATGCTCAAATATGACAGCACACACGGCCAGTTCAAGGGTGAGATCAAGGTTGACGGCAACAACCTGACTGTCAACGGCAAGACCATCCGTTTCCACATGGAGAAGGACCCCGCCAACATCCCATGGAGCGAGACCGGCGCTTACTACGTCGTTGAGTCCACCGGTGTCTTCACCACCACCGAGAAGGCCAAGGCTCACTTGAAGGGTGGAGCCAAGAAGGTCGTCATTTCTGCTCCCTCTGCTGACGCCCCCATGTTCGTTATGGGTGTCAACCACGAGACTTACAAGTCCGACATTGAGGTTCTCTCCAACGCCTCTTGCACAACCAACTGCCTTGCTCCCCTTGCCAAGGTTGTCCACGACAAATTCACCATCATTGAGGGTCTCATGACCACCATCCACTCCTACACTGCCACCCAGAAGGTCGTTGACGGCCCCTCCGCTAA |
| RPB2 | CCGGTGTCTCCCAGGTGTTGAACCGATACACTTACGCCTCCACACTGTCCCATTTGCGTCGAACGAATACCCCTGTTGGTCGAGATGGAAAGCTCGCCAAGCCGCGACAACTCCACAACTCTCATTGGGGTCTTGTCTGCCCTGCCGAGACTCCTGAAGGACAGGCTTGTGGTCTGGTTAAGAACCTGTCTTTGATGTGCTATGTTAGTGTCGGTAGTGACGCTTCTCCCATCATCGACTTCATGACACAACGAAATATGCAACTTCTAGAAGAGTATGATCAGAACCAGAACCCCGATGCCACCAAGGTCTTCGTCAACGGTGTCTGGGTTGGTGTCCATTCCAACGCTCAACAACTTGTCACAGTTGTGCAGGAGCTGCGACGAAACGGAACTCTATCCTATGAGATGAGTCTGATTCGTGACATTCGTGACCGAGAGTTCAAGATCTTTACAGATGCTGGCCGTGTTATGAGACCACTGTTCGTTGTTGAGAACGACATTCGGAAGCCAAACCGCAACCACCTAATCTTCACCAAGGAGATCAGTAACAAGCTCAAGCAGGAGCAACAAGAGACAAGCACACGACAGGGTTGGAGCCAGGATGAAGTCGAATCAGCTACCTACGGCTGGAGAGGTCTTATTCAAGATGGTGTTGTGGAGTACCTAGACGCTGAAGAGGAGGAGACTGCCATGATAACGTTCTCCCCTGAGGATCTGGAGGAGTGGCGAGAGATGAAATTGGGCTTGCCGGCGGCTGAGCGATCCACCGAGGGTGAGCACCGTCTCCGACGCCTCAAGCCACTACCAGACCCCCGCATCCATGCCTATACTCATTGCGAGATTCATCCGGCTATGATTCTCGGTATCTGTGCCAGTATCATTCCCT
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